When All Souls failed to be continued, Aylesworth joined the cast of The American Embassy in the second episode, although she was also credited in the pilot.
Following the cancellation of The American Embassy, Aylesworth auditioned for the part of Nina Myers on Fox Network's 24 but the role went to Sarah Clarke.
Upon being released from 24, Aylesworth was cast as Chandra Moore in CSI: Crime Scene Investigation, the lab's new DNA analyst, and while the role was intended to be recurring, it only lasted for the first episode of CSI's fifth season.
During 24 season 4, it was decided some of the cast from previous seasons would return including Carlos Bernard, Dennis Haysbert, and Reiko Aylesworth.
Aylesworth continued her work in independent productions, filming a small part for Steven Kampmann's independent movie, Buzzkill.
It is not known when Buzzkill will be released as it has not been shown at any film festivals and the movie's website has not been updated since April, 2008.
Soon after NBC cast Aylesworth as Becca Coltrane in the pilot for the series Sherman's March, however, it was not picked up and aired as a standalone television movie.
After her character was written out of One Life to Live, she appeared in the independent film Childhood's End and the final episode of the HBO series Lifestories: Families in Crisis.
Departing from her previous type of characters, Aylesworth played the femme fatale Audrey Levine in the Gideon Raff independent thriller, The Killing Floor.
Her character, Kelly O'Brien, an Army pilot and soldier returning home after being deployed overseas, arrives in Gunnison, Colorado the day before the Aliens and Predator invade the town.
In January 2007, Aylesworth had a guest role in two episodes of the ABC comedy, The Knights of Prosperity, where she played the head of Mick Jagger's security company, Simone Cashwell.
The Knights attempt to seduce her character in order to obtain the necessary security codes to access Mick Jagger's apartment and later trick Simone in order to get her thumbprint which is also needed to open the door.